How can I determine plant extract concentration?
The concentration of the extract is calculated as follows 10gm/100ml = 10×103mg/100ml= 100 mg/ml Different concentrations could be prepared from that stock solution To prepare 5 mg / ml with a...
Plant Extract - an overview | ScienceDirect Topics
The distribution of compounds with respect to number and concentration, in the typical plant extract, follows a curve similar to that illustrated in Fig. 1. There are relatively few compounds present in high concentration and thus accurate analysis of this type of component is likely to present few difficulties because of the limited number of contaminants that can interfere with the analysis.
How can we determine the concentration of an …
Here the word concentration is not suitable but it would be considered 100% plant extract or herbal extract. It still retains some of the solvent (3-1%) which is difficult to be removed. Most of...
Frontiers | Antimicrobial Properties and Mechanism …
Stained cells were aliquoted into control, treatment groups and cell-free extracts. 1 ml of plant extract at a concentration of 20% was added to 1 ml of bacteria or cell-free filtrate. For control groups, 1 ml of PP buffer was added to 1 ml of bacteria or cell-free filtrate.
Antimicrobial activity of some plant extracts …
1-2-2018 · All plant extracts was potentially effective against S. typhi, E. coli, B. cereus and S. aureus implicated in stone spoilage but the extract of Punica granatum was the most effective extract with concentration of 500 mg/ml. Ethanolic P. granatum peels extracts was found to be potentially effective against Micrococcus luteus, S. aureus, Bacillus megaterium and Gram negative bacteria like E. coli …
Cytotoxic Properties of Some Medicinal Plant …
5-11-2013 · The cells were treated with different concentrations of plants extracts including 0.0312, 0.0625, 0.125, 0.25, 0.5, 1, 2.5, 5, 7.5 and 10 mg/ml. Dilution of stock solutions was made in culture medium yielding final extracts concentrations with a final DMSO concentration of 0.1%. This concentration of DMSO did not affect cell viability.
Assessments of Antibacterial Effects of Aqueous …
Based on the mean value zone of inhibition, the plant extracts’ antibacterial activity ability was depending on the concentrations of the plant extracts used. At the concentration of 62.5mg/ml of plant extract, S. aureus had no inhibition zone, whereas other bacteria show little mean zone of inhibition diameter in the same concentration (62.5mg/ml).
Evaluation of Antimicrobial Activity of the …
The plant extracts dissolved in 2.5%dimethyl sulfoxide (DMSO) , which is maximum volume of DMSO that could be used to dissolve solid extracts, were first dilution to the final concentration (2500 μg/ml) for each extract and then serial two fold dilution was made in concentration range 0.6~2500 μg/ml in 10 ml sterile test tube containing 2.5% DMSO.
Interactions between Plant Extracts and Cell Viability ...
Eight concentrations (640 to 5 µg/mL, 100 µL each) of the plant extracts, obtained by two-fold serial dilution in maintenance medium, was added into 96-well plates containing 4 × 104 Vero cells/well. The cells were then incubated for 72 h at 37 °C with 5 % CO 2. After incubation, the
WO2003103371A1 - Cold concentration of plant …
An economical and efficient apparatus and method for concentration of plant extracts is disclosed. The apparatus is in one embodiment a field harvester in communication with a plant cutter and collector which delivers the plant material to a macerator carried by the harvester. From the macerated material, liquid plant values, including enzymes, etc., are selectively obtained by means of, for ...
TOTAL PHENOLIC CONTENT, FLAVONOID CONCENTRATION AND ...
Determination of flavonoid concentrations in the plant extracts The content of flavonoids in the examined plant extracts was determined using spectrophotometric method (QUETTIER et al., 2000). The sample contained 1 ml of methanol solution of the extract in the concentration of 1 mg/ml and 1 ml of 2% AlCl3 solution dissolved in methanol.
Plant extraction - Berkem - Extraction végétale
Plant extraction is a process that aims to extract certain components present in plants. It is a solid/liquid separation operation: a solid object (the plant) is placed in contact with a fluid (the solvent). The plant components of interest are then solubilised and contained within the solvent. The solution thus obtained is the desired extract.
2.0 MATERIALS AND METHODS 2.1 Plant material and extract ...
A stock solution of each plant extracts was prepared by dissolving 100 mg of extract with one ml of their respective solvents (sterile distilled water and 99.9% dimethyl sulfoxide. Ten, 30 l, 50 l of a final concentration of 100 mg/ml have then used to impregnate in sterilized 6 mm blank discs (Oxide, UK). Distilled water and
CONCENTRATION OF DMSO IS TOO HIGH - Cell …
10-10-2006 · In the past Ive used 100% DMSO to dissolve my plant extracts and this posed a problem because it was having a toxic effect on my cells, as a result I had to dilute my extract so much to get it to 0.1% that i had to use such a high volume to arrive at the drug concentration I needed.
for preparing required concentration of plant extract
concentration MIC showed ≤ 6 25 μg ml for almost all test microorganisms and different types of extracts except acetone extract from leaves which exhibited MIC≥ 50 μg ml The present study concludes A vera sap and leaves could be used as medicinal plant with further investigation to determine its toxicity and side effects if any
What is the plant extract - Articles Factory
The plant extract is based on plants as pharmaceutical raw materials in accordance with the need for the use of the extract of the final product, after the physical and stone extraction and separation process, directed to obtain and concentration plants in one or a variety of active ingredients, without changing its active ingredient structure and the formation of the product.
Antimicrobial Activity of Crude Extracts of Some …
Among the plant tested, water extracts of A. discoridis showed the strongest activity against all test microorganisms with the best MIC (4 mg mL-1). The MMC values of the promising plant extracts ranged from 8 to 128 mg mL-1; the lowest MMC for test bacteria and C.
Medicinal Plant Leaf Extract and Pure Flavonoid …
20-11-2017 · On increasing the concentration of plant extract into AgNO 3 solution, a red shift in the absorption band to the longer wavelength (from 430 to 455 nm in case of Tulsi extract …
A Sensitive and Quick Microplate Method to …
Agar diffusion techniques are used widely to assay plant extracts for antimicrobial activity, but there are problems associated with this technique. A micro-dilution technique was developed using 96-well microplates and tetrazolium salts to indicate bacterial growth. p-Iodonitrotetrazolium violet [0 …